Product Info Summary
SKU: | M01608-1 |
---|---|
Size: | 50 µl |
Reactive Species: | Human |
Host: | Mouse |
Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-RAB27A Antibody
SKU/Catalog Number
M01608-1
Size
50 µl
Description
Boster Bio Anti-RAB27A Antibody (Catalog # M01608-1). Tested in WB, Flow Cytometry application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-RAB27A Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01608-1)
Host
Mouse
Contents
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Monoclonal
Clone Number
1590CT813.266.26
Isotype
IgG1,k
Immunogen
This RAB27A antibody is generated from a mouse immunized with a recombinant protein of human RAB27A.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01608-1 is reactive to RAB27A in Human
Applications
M01608-1 is guaranteed for Flow Cytometry, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
24.868kDa
Background of RAB27A
Plays a role in cytotoxic granule exocytosis in lymphocytes. Required for both granule maturation and granule docking and priming at the immunologic synapse.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:2000
FC: 1:25
Validation Images & Assay Conditions
Click image to see more details
Anti-RAB27A Antibody at 1:2000 dilution + K562 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 25 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Click image to see more details
Overlay histogram showing K562 cells stained with M01608-1(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (M01608-1, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was mouse IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Protein Target Info & Infographic
Gene/Protein Information For RAB27A (Source: Uniprot.org, NCBI)
Gene Name
RAB27A
Full Name
Ras-related protein Rab-27A
Weight
24.868kDa
Superfamily
small GTPase superfamily
Alternative Names
GS2; GS2rab-27; GTP-binding protein Ram; HsT18676; Rab-27; Rab27a; RAB27A, member RAS oncogene family; RAB27MGC117246; RAM; RAMras-related protein Rab-27A RAB27A GS2, HsT18676, RAB27, RAM RAB27A, member RAS oncogene family ras-related protein Rab-27A|GTP-binding protein Ram|mutant Ras-related protein Rab-27A|rab-27
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RAB27A, check out the RAB27A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RAB27A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-RAB27A Antibody (M01608-1)
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No publications found for M01608-1
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